Composite

Part:BBa_K4577007:Design

Designed by: Ma Bourgeois   Group: iGEM23_Ionis-Paris   (2023-10-06)


Hao gene with the promotor, terminator and the efficient RBS (g10-L) from bacteriophage T7


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1262
    Illegal XhoI site found at 1433
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 92
    Illegal BsaI site found at 322
    Illegal BsaI.rc site found at 191
    Illegal BsaI.rc site found at 758
    Illegal SapI site found at 1399


Design Notes

The codons have been optimised for the Sinorhizobium meliloti Sm1021 strain.

References

[1] Fan Ouyanga, Dan Penga,Xiao Su, Population dynamics and metabolic alternations of AOB in activated sludge under Cu shock loads, Desalination and Water Treatment, october 2020, doi: 10.5004/dwt.2020.26102

[2] Xu Y, Rosenkranz S, Weng CL, Scharer JM, Moo-Young M, Chou CP. Characterization of the T7 promoter system for expressing penicillin acylase in Escherichia coli. Appl Microbiol Biotechnol. 2006 Sep;72(3):529-36. doi: 10.1007/s00253-005-0293-2. Epub 2006 Jan 13. PMID: 16411086.

[3] HiZJU-China, EE²Limination, IGEM competition, 2021.